https://touchingfish.top/en/tags/rna-seq/Recent content in Rna-Seq on TouchingFish.topHugoenSun, 21 Feb 2021 00:00:00 +0000https://touchingfish.top/en/2021/alternative-splice-algorithm/Sun, 21 Feb 2021 00:00:00 +0000https://touchingfish.top/en/2021/alternative-splice-algorithm/<h2 id="1-biological-background">1. Biological Background</h2> <h3 id="11-what-is-alternative-splicing">1.1 What is Alternative Splicing</h3> <p>In eukaryotes, a single gene can produce multiple mRNA isoforms through alternative splicing (AS) -- the process by which different combinations of exons are joined during pre-mRNA splicing. This greatly expands proteomic diversity without increasing gene count.</p> <p>A gene with N exons can theoretically produce up to 2^(N-1) splice variants. In practice, most genes produce 2-10 isoforms, but some (e.g., Drosophila Dscam) can produce tens of thousands.</p>https://touchingfish.top/en/2019/anguilla-japonica-deg-analysis/Thu, 15 Aug 2019 00:00:00 +0000https://touchingfish.top/en/2019/anguilla-japonica-deg-analysis/<h2 id="abstract">Abstract</h2> <p>This study conducted differentially expressed gene (DEG) analysis on three key tissues (brain, gonad, pectoral fins) of Japanese eel (<em>Anguilla japonica</em>) under seawater (SEA) and freshwater (TAP) conditions, to elucidate the transcriptomic response mechanisms during salinity adaptation.</p> <p>Key findings:</p> <ul> <li>Global analysis (controlling for tissue effects) identified 422 DEGs (303 upregulated, 119 downregulated)</li> <li>The brain was the most salinity-sensitive tissue, with 500 DEGs identified</li> <li>Eight core response genes were significantly differentially expressed across all three tissues (intersection), all upregulated in the seawater environment</li> </ul> <p>Main conclusions:</p>