https://touchingfish.top/en/categories/bioinformatics/Recent content in Bioinformatics on TouchingFish.topHugoenFri, 21 May 2021 00:00:00 +0000https://touchingfish.top/en/2021/alternative-rna-processing/Fri, 21 May 2021 00:00:00 +0000https://touchingfish.top/en/2021/alternative-rna-processing/<p>I received a livestream notification about the &quot;Yunnan Butterfly Explosion&quot; the moment I got to work. I'd been intrigued ever since seeing it on trending topics earlier — back in college, I followed two professors into the mountains to observe butterflies. One was a species identification expert, the other an entomologist. Both were inspiring mentors I deeply respect.</p> <p>The more I think about butterflies, the more fascinating they become. Caterpillar and butterfly — same genome, yet two radically different lives. The genes are not replaced; the same genetic information is simply &quot;interpreted&quot; differently during metamorphosis. It is as if the same transcript gets spliced under a different scheme, emerging with entirely new form and function. This brings to mind an ancient saying —</p>https://touchingfish.top/en/2021/alternative-splice-algorithm/Sun, 21 Feb 2021 00:00:00 +0000https://touchingfish.top/en/2021/alternative-splice-algorithm/<h2 id="1-biological-background">1. Biological Background</h2> <h3 id="11-what-is-alternative-splicing">1.1 What is Alternative Splicing</h3> <p>In eukaryotes, a single gene can produce multiple mRNA isoforms through alternative splicing (AS) -- the process by which different combinations of exons are joined during pre-mRNA splicing. This greatly expands proteomic diversity without increasing gene count.</p> <p>A gene with N exons can theoretically produce up to 2^(N-1) splice variants. In practice, most genes produce 2-10 isoforms, but some (e.g., Drosophila Dscam) can produce tens of thousands.</p>